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The ratio of fibroin to sericin in the middle silk gland of Bombyx mori and its correlation with the extensional behavior of the silk dope

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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8

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Protein Science, Volume 33, issue 3

Abstract

Understanding how native silk spinning occurs is crucial for designing artificial spinning systems. One often overlooked factor in Bombyx mori is the secretion of sericin proteins. Herein, we investigate the variation in amino acid content at different locations in the middle silk gland (MSG) of B. mori. This variation corresponds to an increase in sericin content when moving towards the anterior region of the MSG, while the posterior region predominantly contains fibroin. We estimate the mass ratio of sericin to fibroin to be ~25/75 wt% in the anterior MSG, depending on the fitting method. Then, we demonstrate that the improvement in the extensional behavior of the silk dope in the MSG correlates with the increase in sericin content. The addition of sericin may decrease the viscosity of the silk dope, a factor associated with an increase in the spinnability of silk. We further discuss whether this effect could also result from other known physicochemical changes within the MSG.

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Publisher Copyright: © 2024 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.

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Välisalmi, T & Linder, M B 2024, 'The ratio of fibroin to sericin in the middle silk gland of Bombyx mori and its correlation with the extensional behavior of the silk dope', Protein Science, vol. 33, no. 3, e4907. https://doi.org/10.1002/pro.4907

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